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Histological analyses of the quadriceps demonstrated that PF-879-mediated increases in mean fiber area and the percentage of larger fibers (Figure 2A C).

Furthermore, the mean fiber area in the vastus lateralis muscle of Aymara Bolivian natives residing in a La Paz at 4100 m is significantly smaller than in native European lowlanders [5].

Mean fiber area and fiber type distribution will be analyzed as described by Andersen & Aaagaard [ 5].

Sample size is chosen to ensure significant muscular hypertrophy, evaluated by mean fiber area, in CON, among whom a 15% increase is estimated according to earlier studies.

Primary outcome: mean fiber area and fiber type composition measured by histochemical analyses, satellite cells and levels of protein and mRNA expression of intracellular mediators of protein turnover.

For immunohistochemistry, cross-sections were cut in a cryostat microtome (CM3050, Leica Biosystems GmbH, Wetzlar, Germany), and the samples were stained for dystrophin, SC71 (fiber type II) and CD56/NCAM for analyses of mean fiber area, fiber type distribution and satellite cells; DAPI was used to mark myonuclei (for more details regarding antibodies and procedures, see [ 38- 40]).

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Similar(53)

Average fiber area was quantified based on the average area of ∼20 fibers for each fiber type per image [14].

This result is in agreement with a recent study in which ACE031 (the humanized analogue of ActRIIB-mFc) treatment increased the mean fiber cross-sectional area in plantaris, a predominantly type II muscle, and as well as type I and type II fibers in the soleus muscle [26].

These data describe a pattern of improved precision in estimating mean fiber cross-sectional area as sample size of fibers measured increases to at least 150 in this rat model.

CNF A and CNF C have a higher surface area, a lower mean fiber diameter, and a higher presence of structural defects than CNF B and CNF D samples (Table 1).

Common for CNF A and CNF C is a higher metal content, about twice as large relative surface area, a lower mean fiber width, a higher proportion of open channel fibers, and a tendency towards a higher number of defective sites compared to CNF B and CNF D (Table 2).

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