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The influence of the processing parameters, including type and amount of nanoparticles in the composite, on the mean fiber size and size distribution was studied.
Accordingly, the comparison of fiber CSAs between patients and controls would possibly show a lower mean fiber size of the patient group and indicate muscle atrophy.
Thereafter the mean fiber size of each muscle CSAs were calculated.
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This means that fiber size and interconnectivity have an opposite correlation.
However, the mean cellular viability rose from 50 to 110% compared to that belonging to the control even at narrow distributions of mean fiber diameter and pore size from 170 to 320 nm and 330 to 790 nm, respectively.
Whereas most fibers tend to cluster within a size range close to the mean in wild type mice, fiber size is more broadly distributed in Myo-CELFΔ-370 mice, with a larger percentage of fibers falling at the ends of the spectrum (Figure 3F).
These data describe a pattern of improved precision in estimating mean fiber cross-sectional area as sample size of fibers measured increases to at least 150 in this rat model.
Sample size is chosen to ensure significant muscular hypertrophy, evaluated by mean fiber area, in CON, among whom a 15% increase is estimated according to earlier studies.
Two parameters were measured; fiber size represents the relative "length" of each skeletonized fiber, and density represents the mean grey value of the fluorescence intensity of each image.
Although our fiber estimates cannot be extrapolated to very aged or diseased rat models with accelerated muscle wasting or, to a lesser extent, middle-aged female rats, our results suggest that the reliability of mean FCSA estimates based on a subset of <150 fibers would be poor due to heterogeneity in fiber size.
The fiber size changes a lot from micrometers to nanometers.
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