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The maximum fluorescence wavelength of the final cyanine dyes shifted red compared with those of Cy3 and TO alone.
The QD conjugates were characterized by spectrofluorimetry and 1% agarose electrophoresis, presenting a blueshift in the maximum fluorescence wavelength and a slow electrophoretic mobility (Figure 1).
The absorbance spectrum, fluorescence spectrum and images of QDs before and after the coupling with SPA under UV irradiation is shown in Fig. 4. It is showed that the QDs could be excited effectively under ultraviolet band and their maximum fluorescence wavelength was at around 610 nm before conjugation and shifted to around 596 nm after conjugation.
The fluorescence spectra and images of QDs before and after the coupling with sheep-anti-human secondary antibodies under UV irradiation are shown in Fig. 4. According to our results, the QDs have strong fluorescence, and the maximum fluorescence wavelength for CdTe was at approximately 545 nm.
MBP exhibits a quenching of fluorescence and small shift in the maximum fluorescence wavelength upon maltose binding owing to the presence of several tryptophans in the vicinity of the maltose-binding pocket, a behavior that RG13 shares.
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The maximum fluorescence wavelengths emitted were, respectively, 575 and 640 nm with the corresponding peak width at half height of 40 and 30 nm.
In the case of the two forms of 4-MU and quinine, the initially set value of the emission wavelength corresponds to the maximum-fluorescence wavelength obtained in a cuvette and in the microfluidic detection cells.
Thus, the detected signal at the microscale was higher, as the maximum-fluorescence wavelengths in the microfluidic cells were closer to the initially set value of the emission wavelength of 545 nm.
The figure showed the fluorescence spectrum of the protein when the excitation wavelength is 280 nm, the maximum fluorescence emission wavelength (λmax) of BSA is about 330 nm.
The maximum fluorescence peak wavelength returned towards the control maximum fluorescence peak wavelength for rabbits fed on HCD + Zn.
Figure 3 shows that the maximum fluorescence peak wavelength shifted towards the visible region for HCD group, and it returned towards the control maximum fluorescence peak wavelength for rabbits fed on HCD + Zn.
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