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Figure 1b shows a magnification of the area with the maximum current amplitude.
n, the number of the channels in the patch, was defined as the maximum current amplitude observed, divided by the unitary current.
In order to estimate the maximum current amplitude, we applied graded mechanical stimuli of increasing intensity until the patch clamp seal became unstable.
From the fitted values of N and g, an estimate of effective Po at a particular voltage was obtained from Po(V) = Imax/ N*g*V), with Imax the maximum current amplitude recorded at that voltage.
The maximum current amplitude of 12.0 mA was programmed in 2.7% of subjects.
by plotting the amplitude of agonist-induced currents (normalized to maximum current amplitude produced by respective agonist for each individual cell) against log agonist concentrations.
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As Imax = i×N×P(O max, where i is unitary current, N is the number of channels, and P(O max is the maximum open probability that can be achieved, a change in any of these parameters will cause maximal current amplitude to change.
Additionally, the decrease in current amplitudes at the end of pulses relative to the maximum current amplitudes was greater for the glycosylated Kv3.1 channel than unglycosylated.
Whole cell current recordings were accepted during those experiments only if membrane seal resistance was ≥1GΩ, maximum current amplitudes were ≥400 pA, and minimal cell capacitance was displayed after compensation.
Maximum lightning current amplitude that does not cause flashover across insulator string is estimated in response to first and subsequent lightning strokes with three different flashover models using several electrogeometric models and simulations in EMTP-ATP.
DOI: http://dx.doi.org/10.7554/eLife.10988.005 Exocytosis triggered by voltage-activation of Ca2+ channels from -80 to -10 mV (a voltage at which Ca2+ current amplitude is maximum, Figure 3A) also showed maximum facilitation when intracellular pressure was increased from 310 to 390 mOsm.
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