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After 72 hours of fermentation, maximum biomass production (6.55 g/l) was obtained with the yield (0.17 g/g) and productivity (0.09 g/l/h).

Accordingly, the maximum biomass production was calculated 4.87, and 1.51 g/L respectively.

The reactor must be operated at maximum biomass production rate by manipulating the feed rate on the basis of a (biomass or substrate) measurement.

The pattern of substrate catabolism forms a substrate assimilation fingerprint which is useful in selecting media components for media optimization of maximum biomass production.

For P. putida S12 the biomass-to-substrate yield, maximum biomass production rate and substrate uptake rate were consistently higher on crude glycerol.

The optimal medium for maximum biomass production was glucose 10%, corn flour 3%, glutinous rice flour 3%, fish peptone 0.2%andKH2PO40.3%.3%, it was incubated at 23 ± 2 °C with agitation rate of 150 rpm.

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The maximum biomass yield and CMCase production (450 mg g−1 and 9.1 U mL−1 respectively) was obtained at 117.2 and 468.8 µg Ni2+ mL−1 of the nickel ions, respectively (Fig. 4).

Since production of acetoin and 2,3-butanediol is generally accepted to be a growth-associated phenomenon, the conditions for the maximum product formation must approximately be the same as those for the maximum biomass yield [ 31].

As with most FBA calculations, we chose maximum biomass (BM) production as the objective function by formulating a simple BM reaction composed of carbohydrates and nucleotides (see Figure 1).

This implies that the sole N reserves in soil with lower N rate of application could have been sufficient for maximum shoot biomass production of common bean at R3 stage.

To obtain a realistic upper value for the current that can be extracted from a microbe, 5% of the maximum theoretical biomass production rate was chosen as the viability threshold for computational identification of the lowest growth rate.

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