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Under optimum culture conditions, the batch culture exhibited a maximum biomass concentration of 12 g/l and GA3 production of 1 g/l in 170 h.
The results showed that when the Box-Behnken design was applied, the maximum biomass concentration was 4.78 times higher than that from unoptimized condition.
This, allowed manual base only pH control to be implemented in microwell fermentations which enhanced both the maximum specific growth rate and the maximum biomass concentration.
In the bubble column photobioreactor, on the other hand, the maximum biomass concentration reached was 1.4 g/L, with an overall biomass productivity of 0.06 g/L day.
In the absence of culture aeration in the conical flasks, a maximum biomass concentration of 3.20 g/L was achieved under alkaline conditions with initial pH of ∼9.
Hybrid ANN-GA predicted the maximum biomass concentration (12.25 g/L) at the optimum level of medium variables: sucrose, 35.27 g/L; urea, 1.55 g/L; and TE solution, 0.42 ml/L.
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Finally, maximum biomass concentrations of 48 g L−1 dry cell weight and maximum space-time yields of 2.10 g L−1 h−1 were reached.
A. wieringae and S. ovata showed by far the highest growth rates and maximum biomass concentrations among the acetogens under study.
The maximum biomass concentrations in type-a, type-b, and type-c reactors were 42.9% (1.3 g L−1), 31.9% (1.2 g L−1), and 20.9% (1.1 g L−1) higher than that in the control reactor (0.91 g L−1), respectively, at an aeration rate of 1.0 vvm.
The maximum biomass concentrations as well as their μmax values were found to be very similar for all strains.
An integrated statistical design, which incorporated single factor design, response surface methodology (RS M and weighting coefficient method, was used to determine the optimum hydrolysis conditions leading to maximum biomass, ethanol concentration and starch utilization ratio.
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