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The maximal aggregation and slide platelet aggregation test (SPAT) induced by the inducer were calculated as indices of the aggregation function of platelets.

In patients with sepsis, the platelet first wave of aggregation (in response to ADP), maximal aggregation and secretion of ATP (in response to ADP, collagen, U46619 and thrombin receptor activating peptide) were significantly lower than those of controls (Table  2).

We observed indirect effects of air pollution on platelet aggregation, represented by a positive significant association between 5 μmol/L ADP-induced maximal aggregation and PM10 concentrations for time lag I72 96 (+8%/IQR, p < 0.01).

The significant associations between various air pollutants and increased maximal aggregation and late aggregation for time lags within 48 96 hr before blood sampling suggest that exposure to air pollution indirectly increases blood thrombogenicity.

Platelet maximal aggregation and secretion (in response to ADP, collagen, thromboxane A2 analogue U46619 and thrombin receptor activating peptide) and first wave of aggregation (in response to ADP) were measured in healthy volunteers and patients with sepsis (within 48 hours from admission to intensive care).

We induced platelet aggregation by 5 and 2.5 μmol/L ADP Sigma Chemical Co.., St . Louis MO, USA) and determined maximal aggregation and late aggregation (residual aggregation at 6 min after the maximum representing platelet aggregate stability) by recording for 10 min on a four-channel optical aggregometer (Chrono-log, Kordia Life Sciences, Leiden, the Netherlands).

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Single-linkage clustering generates maximal aggregation of sequences, and will bring together both closely related paralogs and their alleles (Dlugosch and Bonin 2012).

Platelets of patients with sepsis were generally hypo-responsive to exogenous agonists, both in terms of maximal aggregation (P < 0.001) and secretion (P < 0.05).

The "Area under the curve" (AUC in AU*min) is an integrated measure of the velocity (AU/min) of aggregation and is reported to reflect maximal aggregation.

Maximal aggregation diminished in line with secretion and granular content, as it does during inherited defects of δ-granules [ 40, 41].

The PKCε-null platelets showed a minor delay in the onset of aggregation and a minor reduction in dense granule secretion to concentrations of thrombin (0.03 units/ml) and PAR4 peptide (100 µM) that induces near maximal aggregation in litter-matched control platelets (Fig 5A and not shown).

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