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All proteins used were subjected to an extra gel filtration step and dialysis overnight in TB buffer with 15% glycerol to remove possible aggregation and ensures buffer matching.
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Differential scanning calorimetry (DSC) was carried out for selected mutants (ΔF4 and ΔW6) to look into possible aggregation of the protein, and R-BSX was used as a control.
In order to visualize and investigate Aβ deposition and possible aggregation in the brain, Congo Red dye was injected in the Cisterna Magna (Additional file 2: Figure S2).
Low expression and possible aggregation of PS11-scFvFc-gp41 PS11-scFvFc-gp41 PS11-scFvFc-gp41 folding, as the natural Fc moiety forms dimers, while gp41 forms trimers through its TM.
For the M284K variant it would be especially interesting to quantify synthesis level, stability, localisation, and possible aggregation, to see if higher levels of soluble, active M284K-hGALE protein might be detected in a mammalian system.
On the other hand, our DSF results suggested a possible aggregation tendency of the larger and more stable C-terminal domain and we investigated this feature in more detail.
In the third stage, the ISMU interviewers visit all possible aggregation centres for immigrants within each municipality and randomly meet the potential interviewees.
Arrow considered a class of possible aggregation methods, which he called social welfare functions, and asked which of them satisfy certain axioms or desiderata.
Then there are lk possible aggregation rules: the relevant table has k rows, and in each row, there are l possible ways of specifying the final entry (the collective decision).
Thus the number of possible aggregation rules grows exponentially with the number of admissible profiles and the number of possible decision outcomes.
The second micronaire hotspot and the FS hotspot overlapped, indicating a possible aggregation of fiber quality QTL hotspots.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com