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As shown in Fig. 2A and 2B, mating efficiency was higher in tor2-51 pka1Δ than in tor2.51 and was almost completely abolished in tor2.51 cells cells.

Male mating efficiency was assessed based on two of the assays described above.

The mating efficiency was high (10−1 transconjugants/donor) and liquid mating was as efficient as surface mating.

The mating efficiency was calculated as a CFU of transconjugants per CFU of recipient.

The mating efficiency was determined as 2.45%.

The mating efficiency was calculated by dividing the number of diploid cells by the number of cells carrying a hygromycin B-resistant marker.

Similar(54)

The reduction in mating efficiency is particularly pronounced in N2 (see results from the mating behaviour experiments above).

We hypothesize that the low mating efficiency is largely due to defects in male-specific somatic structures.

Another potential explanation for the lower mating efficiency is that sperm activity in 2-day-old sir-2.1 0) sir-2.1 0defective.

As measured by mating efficiency, silencing is indistinguishable between wild type and mutants lacking any of the four HST genes (Brachmann et al., 1995; Yang et al., 2008).

3) In the case of one of our assays, male-hermaphrodite precedence tests using dpy-4 recipients, differences in mating efficiency would be expected to contribute to the magnitude of the phenotype we observe; but our conclusions do not rely on this assay alone.

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