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Male mating behaviour was also strongly age dependent (Fig. 2).
Male mating behaviour was well timed to female ovulation, suggesting that males had accurate information about this.
Male mating behaviour was observed every 30 min within the first 6 h of each day after a set of four virgin females had been added to the mating cage.
To check whether mating behaviour was associated with the survival of adult moths for three days, we also recorded adult mortality daily for three days following the mate choice experiments.
Furthermore, compared with the dioecious species Caenorhabditis remanei, mating behaviour was severely compromised in the N2 strain, i.e. males often fail to find hermaphrodite mates, possibly due to limited production and/or degeneracy of the hermaphrodite's sex pheromone [[ 7, 12] but see, [ 13, 14]].
Mating behaviour was observed with the use of a red LED light (Sigma LED safety light).
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Mate preferences, and indeed mating behaviour, are strongly influenced by morphological traits exhibited by a potential partner [1], [2].
In the bumblebee Bombus terrestris (with CSD), mating behaviour is also affected by relatedness of partners [40].
In no-choice trials, various aspects of mating behaviour were observed and recorded, and for all experiments, an index of sexual isolation was calculated based on the relative numbers of matings between flies in different pair combinations using the program JMating [ 57, 58].
Interfering with mating behaviour is an attractive idea, which has shown success with many agricultural pest species, but the longevity of bed bugs (months to a year or more) and our lack of tolerance of their repeated biting, makes the concept much more challenging.
In many animal species, plastic or flexible mating strategies exist, and plasticity in mating behaviours is usually an adaptive response to varying natural and social contexts [12] [14].
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Justyna Jupowicz-Kozak
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