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However, few of the single colony isolates from the diploid ctf4Δ strain were totally converted to either a MATa or MATα phenotype (2 and 1% respectively) suggesting that for the majority of cells mating type was unaffected and it is the loss of CTR4 that is responsible for DOX sensitivity in this strain.
Moreover, mating type was completely linked to RFP in 20 tetrads.
Phylogeny was assessed using partial β-tubulin and calmodulin gene sequencing, and mating type was determined by PCR.
Of the E. typhina strains that were subjected to analysis, E. typhina 9340 and E. typhina MAFF (AB161000.1) display the MTB idiomorph, while the opposing (MTA) mating type was observed in E. typhina E8.
Our results may support the conclusion of host-dependent effects upon hybrid fitness, where backcrossing that was homospecific for the M. lychnidis-dioicae mating type was significantly favored on S. latifolia but not on S. colorata, consistent with the expectation that host adaptation to S. latifolia is an extrinsic post-mating factor [ 42].
Although the precise genetic control and heritability of Types I III remain obscure, each morph is presumed to be genetically encoded and unresponsive to environmental influences, as within-individual variation in mating type was not observed under controlled conditions [ 47, 48].
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Thus, simple, rapid, and efficient methods for bulk isolation of pure ascospores sorted by mating type are needed.
The only recombination gene that is clearly regulated at the transcriptional level by mating type is RDH54.
In S. cerevisiae, mating type is determined by the expression of MAT a or MATα mating-type genes residing at the MAT locus.
Our studies revealed that mating type is directly related to gynostemium structure, most often involving the presence or absence of a physical barrier termed 'rostellum'rostellum
Using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species Ophiostoma quercus.
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