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Each mating pair was placed in a small plastic container (7×7×5 cm) with dampened paper towel lining the base.
Each individual mating pair was observed for a period of 90 minutes until copulation was observed.
If copulation was ongoing at the 90 minute mark, the mating pair was observed until copulation ended.
Later the same day (between 13.00 and 16.00 h) a virgin male was introduced to the cage and the mating pair was left undisturbed for 24 h.
We selected several mating pairs from the TAB-5 pool, and the most robust mating pair was chosen as the founding pair for NHGRI-1.
Later the same day (between 13.00 and 16.00 h) a non-sibling virgin male was introduced to the cage and the mating pair was left undisturbed for 24 h.
Similar(53)
We found that the mean litter size, 3.3±1.4, of Coil −/− mating pairs was significantly smaller than that of wild-type mating pairs, 6.9±2.2 (Figure 1A).
Accounting for these pseudo mating pairs was necessary to ensure Mendelian segregation at fog-2 under selfing.
Non-mating flies were removed and mating pairs were left until the next day.
During the rainy season, males become territorial, and mating pairs are established.
In H. argenteocinereus, paternity analyses demonstrated that burrow systems of mating pairs were several hundred meters away from each other in some cases.
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