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The conserved markers we designed and characterised are very convenient to use.
In addition to the available microsatellite markers, we designed new microsatellite markers as shown in Supplementary Table S1.
To exploit these polymorphisms in the development of molecular markers, we designed primer pairs based on genomic sequences flanking carefully selected mPing insertions (Fig. 2A).
Due to difficulties in repeating the published multiplex protocol, and to enable the analysis of additional markers, we designed novel primers for MLVA amplification.
We felt that if we could replicate the known primate phylogeny using the markers we designed then we would have proof of principle that this type of marker was useful for phylogenetic analysis.
As an alternative approach, we used positional cloning to delimit the SVR3 locus to a ~123 kb interval on chromosome 5 using a series of molecular markers we designed using the Cereon genomics Indel and SNP databases.
Similar(51)
To determine whether the SSRs varied in length, and would therefore provide a useful marker, we designed flanking primers and determined the length of each SSR by capillary electrophoresis.
To allow for simple amplification of the marker, we design specific primer set for this part of LSU rDNA.
To assess the reproducibility of measuring inflammatory markers in bile, we designed a methods study of 69 multiplexed immune-related markers measured in bile obtained from gallstone patients.
Using these two functional markers along with the already reported functional PCR-based marker for Xa21 (pTA248), we designed a single-tube multiplex PCR based assay for simultaneous detection of all the three major resistance genes and demonstrated the utility of the multiplex marker system in a segregating population.
In this study, in addition to the characterization of EST-derived SSR markers in cultivated sweetpotato, we designed and validated 1,060 SSR markers in two parents of our mapping population.
Related(20)
beacons we designed
markers we developed
parameters we designed
microsatellites we designed
markers we develop
markers we genotyped
markers we tested
markers we compared
markers we assessed
markers we studied
markers we stained
markers we extracted
markers we reconstructed
markers we hit
markers we used
markers we screened
markers we suspected
markers we were
markers we increased
markers we found
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