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Exact(6)
Sequence-tagged site (STS) markers for candidate genes were designed based on polymorphisms detected in the parents.
Genetic markers for candidate genes related to fruit texture were then located on the map (Supplementary Table S1).
DC and CK analyzed the chemical datasets, constructed genetic maps, performed statistical and QTL analysis, developed markers for candidate genes and wrote the manuscript.
Genetic markers for candidate genes targeted for mapping were either PCR-based (StTLRP [gb|GU233535], micro.17361.c1) http://pgrc.ipk-gatersleben.de/poci or CAPS-based (bch [gb|GU233534]), and integrated using the same linkage mapping software.
Finally, because the microsatellites documented here are in or close to genes (given that the majority of contigs either align with genes or map to within 5,000 bp of a gene (Table 5)), they are ideal markers for candidate gene studies or other population genetic studies requiring a modest number of variable markers which are uniformly distributed across the genome.
A total of 10,971 bp were aligned over all loci for the 20 genotypes, the length of sequence alignment for each gene was about 1 kb (904 1085 bp), which is used to develop markers for candidate gene allele tracing in selection experiments (unpublished results).
Similar(54)
We have described polymorphic markers for the candidate genes before (van den Berg et al. 2003b, 2004, 2005).
Then, we perform five F2 association experiments using markers for 21 candidate genes previously identified in D. melanogaster.
Putting forward new genetic markers for these candidate genes and testing them in turbot families will be essential to go further in the functional explanation of the detected associations, as in the previous marker-associated approach.
The Carthagene software [ 12, 14, 54] was used to compute efficiently marker ordering for candidate maps with reference maps.
LD maps for SNPs within a single gene are available publicly and provide important insights into choosing marker SNPs for candidate gene analysis.
Related(18)
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