To develop markers for the construction of walnut genetic map and anchoring walnut FPC contigs on it, a total of 54,912 BESs from walnut cv 'Chandler', a major walnut scion cultivar grown in California, have been generated by Sanger sequencing [ 10].
Therefore, a high-density genetic linkage map for walnut is required that comprises a large number of markers with sufficient sequence information.
The DNA extraction method based on CTAB phenol chloroform was best for walnut.
The rate of polymorphism increased with the repeat length, therefore, class I SSRs are useful source of polymorphic DNA markers in walnut.
In the present study, according to the high-density genetic map for walnut, one QTL for walnut anthracnose resistance was identified.
Our results provide an important theoretical basis for walnut molecular assisted breeding and resistance gene identification.
We first constructed a genetic map for walnut, a woody perennial.
One QTL for walnut anthracnose resistance was identified based on the map.
In recent years, a bacterial artificial chromosome (BAC) library was used to develop single nucleotide polymorphism (SNP) markers in walnut [ 19, 2].
