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Using PD as a neurocognitive marker, we tested five predictions derived from the decoupling hypothesis of offline thought.
Utilizing the GFP marker, we tested the recombination efficiency by flow cytometry.
To validate methylation in NT5E as an independent prognostic marker, we tested a second novel epigenetically regulated gene, RasL12.
To confirm the validity of TfRs as a recycling endosome marker, we tested another recycling endosome protein, Rab11, and found significant co-localization between mCherry-tagged Rab11 and TfRs confirming that TfRs are largely in REs in dendrites.
To validate the prognostic value of this marker we tested whether: 1. high initial EBC 8-IP predispose to more severe disease; 2. low initial concentrations increase a chance of early remission; 3. remissions are connected with the decrease of EBC 8-IP.
As the ISBP markers are a popular type of marker, we tested the utility of in silico anchoring also for this type of markers and found that many repetitive sequences, which are not present in high copy number in wheat genome could be assigned to individual BAC clones (data not shown).
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Using axonal and dendritic markers, we tested whether cytoskeletal changes could cause similar transformations, and found that actin depolymerization induced multiple axons in unpolarized neurons.
Of the eight plant DNA markers we tested (rbcLa, rpoC1, rpoB, matK, ycf5, trnL, psbA-trnH, ITS), matK and ITS had a low rate of sequencing success.
Considering the 10 selected genes as representative prognostic molecular markers, we tested whether expression of the genes or their subsets could predict the duration of survival in an independent cohort.
To look for candidate nuclear DNA markers, we tested 17 different nuclear loci.
For 16S rRNA markers, we tested only those with mean relative abundance >0.01% but for metagenome markers used all markers.
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