Exact(24)
The mitochondrial marker we used here would also be useful to identify between-subclades female gene flow, but a nuclear marker is necessary to track male exchanges.
As a selection/counter-selection marker we used the gene coding for the orotidine-5-phosphate decarboxylase (Boeke et al. 1984) (pyrG, from Aspergillus oryzae).
As endosomal marker we used an antibody raised against Hrs [17].
As a membrane marker we used CD8-GFP (detected with gold-labeled anti-GFP) expressed from a transgene.
As a neural crest cell marker, we used AP2α (Activating Protein-2α), a transcription factor involved in face and limb morphogenesis [44] [47].
For model validation in the absence of any other pertinent marker, we used the clinically measured changes in prostate-specific antigen (PSA) levels as a correlate of tumor burden.
Similar(36)
No matter which marker we use the numbers of naïve cells dramatically decreases with ageing, mainly among CD8+ cells.
In this study, to further evaluate the prognostic usefulness of the markers, we used a receiver operating characteristic (ROC) curve (Heagerty et al, 2000).
In addition, the intron markers we used were originally selected for their high rates (together with other useful properties for phylogenetic reconstruction) [ 35].
To correct for multiple testing (four serological markers), we used the Bonferroni approach to determine the significance threshold (0.05/4 = 0.0125).
The transcriptional chromatin markers we used included both transcriptionally active (acetyl-H3 and dimethyl-H3K4) and inactive (trimethyl-H3K9) markers.
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