In order to determine the number of cells expressing ductal markers, we counted the total number of cells in randomly chosen fields and calculated the percentage of those cells expressing the markers CK7, CK20, PNA and OV6 (Figure 3B).
For quantitative analysis of synaptic marker expression we counted the number of granules of reaction product after the synaptophysin staining.
For the single marker test SM, we counted a gene detection for any gene containing a marker below the P-value threshold.
Using GLS (Griffonia simplicifolia lectin), a specific marker for endothelial cells, we counted 21.2 ± 1.6 (n = 7) capillaries in wild-type and 16.4 ± 1.0 (n = 7) in femoral sections of Dnmt2−/− mice (Fig 1B and C).
After having removed all markers with suspect genotypes (77), we counted a total of 113 unambiguous instances of two simultaneous recombination events on a chromosome.
The melting spring snow made the middle section a slog through hundreds of metres of slushy moguls, where we counted down the piste markers, before hitting the last, almost horizontal section, where the late-season snow slowed us again.
Based on these data, we counted the number of markers showing informative genotypes.
Resolution in kb was also determined by dividing by the size in base pairs of each region in Btau_4.0 by the number of markers with unique vectors (clustered markers counted as single markers).
The engrafted cells in each section that were positive for both Venus and each cell type-specific marker were counted (n = 4).
Three brains per genotype per marker were counted.
