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The gene encoding Sp17 is mapped by sequence database to chromosome 11 in human.
Because miRNA probes from the various platforms were based on different MiRBase databases (Table 1), probes were first mapped by sequence.
In silico analysis shows the number of bases and percentage of genome which can be uniquely mapped by sequence reads (equivalent to theoretical maximum).
To ensure the correct annotation, probesets from both microarray platforms, as well as MPSS tags, were mapped by sequence alignment to a human transcriptome (HTR) database that was previously used for a multiple platform comparison study [ 20, 21].
The carotenoid genes mapped by Just et al. [ 8] are not easily transferred to other maps with different genetic backgrounds, since they were mainly mapped by sequence detection of single polymorphism nucleotides (SNPs), rather than by fragment length polymorphisms, and because sequence conservation was very high (i.e., lack of polymorphism) in some carotenoid genes [ 8].
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To identify potential miRNA genes, the MIREAP algorithm [19] was then employed to obtain all candidate precursors with hairpin-like structures that were perfectly mapped by sequencing tags (see Methods).
To identify potential miRNA genes, the MIREAP algorithm (http://sourceforge.net/projects/mireap) was employed to obtain all candidate precursors with hairpin-like structures that were perfectly mapped by sequencing tags.
The locations of 234,152 and 53,556 integration sites, respectively, were mapped by sequencing.
PCR products were precipitated, visualized with the [P]-extension primer and the 3' termini were mapped by sequencing as described below.
The products were analyzed in the polyacrylamide gel and the 5' termini were mapped by sequencing as described in Sequencing of the PCR products below.
Eight of the 12 lines that could not be mapped by sequencing show YFP expression patterns consistent with protein traps and five are lethal or semi-lethal.
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