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We addressed this possibility by including as queries for our TBLASTN analysis of the bacterial genomes, various superfamily member proteins, which could reveal false positive PGM identification or cases where functional assignment by sequence similarity is ambiguous.
This broad spectrum of substrates and mechanisms has challenged automated function assignment by sequence identity.
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Its core consists of manually curated alignments and of knowledge-based rules that enhance assignments made by sequence similarity.
The meta-stable conformations of the molecules are not considered during sequence assignment by the single state sequence design algorithm (panel 2 in Table 1).
SNP chromosomal assignment by turkey linkage, turkey sequence and chicken sequence maps NA = Not Aligned Recombination rate of turkey chromosomes varied from 1.6 to 17.2 cM/Mb.
Even for this study's much longer Sanger sequencing reads, cDNA assignment was sometimes complicated by sequence differences introduced by RT-PCR/sequencing errors, SNPs, or artifactual cDNA sequences due to recombination of transcripts from different HML-2 loci during cDNA generation (Flockerzi et al. 2007) resulting in mismatches of cDNA sequences to the genomic reference.
Therefore, sequence assignment by ROSETTA design is evolutionarily uncommitted, and the sequence conservation we observed in the alignments must derive from the structural and chemical constraints imposed by the backbone coordinates fed into to the program.
We describe an algorithm and the accompanying software, taxator-tk, which performs taxonomic sequence assignment by fast approximate determination of evolutionary neighbors from sequence similarities.
The pathway assignments were carried out by sequence searches against the KEGG database, also using the BLASTX algorithm with an E-value threshold of 10-5.
The pathway assignments were carried out by sequence searches against the KEGG database [ 54], also using the BLASTX algorithm with an E-value threshold of 10-5.
Randomisation will be performed by a computer-generated random assignment sequence by a statistician and opaque, numbered, tamperproof envelopes containing assignment will be prepared in advance.
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