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The amplicons were evaluated by sequence clone libraries and phylogenetic analyses.
The respective subtypes or cardiotropic viruses can be evaluated by sequence analysis.
Only one K. pneumoniae isolate was evaluated by sequence analysis because all three of the K. pneumoniae isolates showed the same enzymes on the IEF gel (Table 2).
Closer examination of some of the cases indicated that this can often be explained by the fact that the ohnolog is actually in an early stage of pseudogenization through gradual decay of its coding sequence, as evaluated by sequence alignment.
For each mappable sequence between 16 and 28 nt and with 5 counts or above, hairpin folding was evaluated by sequence analysis to identify the presence of a stem loop using the RNA secondary structure prediction tool in the CLC Genomics Workbench.
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The quality of the pool was evaluated by sequencing 20 clones from the mutagenized DNA library.
Errors due to the analytical procedure were evaluated by sequencing 42 control clones obtained from an already cloned and sequenced HPRT segment.
Candidate genes were evaluated by sequencing the whole transcript including exon-intron boundaries and potential promoters in the parental lines of the HS mice.
Such potential genomic diversity may be essential to understanding the fitness of relative genotypes within the subclades, and this should be further evaluated by sequencing and comparing more representative strains.
The methylation status of each CpG site was quantitatively evaluated by sequencing multiple clones.
The protocol was evaluated by sequencing sub-colony structures from human embryonic stem cells containing 150 to 200 cells.
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