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As in Figure 6, individual cells representing the staining patterns observed in each image were manually cut from the images, and labeled based on their apparent organelle (o), membrane (m) or nuclear (n) staining patterns.
To assemble figures, individual cells representing the staining patterns observed in each image were manually cut from the images, and labeled based on their apparent organelle (o), membrane (m) or nuclear (n) staining patterns.
Whole tissue mounts were manually cut from excised tissue before being placed in a well and treated identically to cultured cells.
The 68 differently expressed spots were manually cut from gels and characterized by LC ESI MS/MS.
The somata and any obvious artifacts were manually cut from each image, and then we obtained a count of the black pixels.
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For protein identification, spots were manually cut out from SDS-gels, transferred to 96-well microtiterplates (MTP) and processed on an automatic Ettan TA Digester (GE Healthcare, Freiburg, Germany).
Circled areas consisting of approximately 75% LCIS cells were manually cut out from the OCT-embedded frozen tissue blocks (Additional file 1), and RNA was extracted from these enriched samples to create the LCIS SAGE library.
We proceeded to manually cut the excess agar from both the base and the ends of the samples.
Protein spots from 2D SDS-PAGE were manually cut and processed using In-Gel tryptic digestion kit (Pierce).
Fresh uncured ham was purchased from a local supermarket at Wuxi, Jiangsu, China, and manually cut into 7 × 8 cm pieces.
Median transverse sections (1.0 1.5 mm thickness) of developing caryopses and matured grain were manually cut with a sharp razor.
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