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The skins were manually separated from pulp, weighed and frozen at −20°C until analyzed.
Briefly, the amnion was manually separated from the chorion, washed extensively in phosphate-buffered saline (PBS) containing 100 U/ml penicillin and 100 g/ml streptomycin, and cut into small pieces.
To maintain pluripotency, colonies were manually separated from feeder cells and morphologically identifiable differentiated colonies.
Oocytes were manually separated from the granulosa cells upon hyaluronidase digestion, to discriminate populations.
Elymus multisetus were identified by leaf and root characteristics and manually separated from B. tectorum roots.
While frozen the berries were manually separated from stems, leaves, insects, and other debris.
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Guar gum is prepared by first drying the pods in sunlight, then manually separating from the seeds.
Intact, manually separated blastomeres from wild type and maternal knockout E-cadherin+/− two- to eight-cell stage mouse embryos were used.
The input for the medium-scale plant in Bali is post-collection, manually separated organic waste from the mixed municipal waste.
Male and female parasites were manually separated and total RNA from the parasites was purified with Trizol reagent (Invitrogen, CA, USA) as described [5], [30].
In this study, we manually separated chorionic large arteries from the chorionic plate that entirely covers the foetal surface of the placenta, which is, in turn, covered by the aminion (Fig. 1A).
More suggestions(15)
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