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Sequences were manually assembled using Vector NTI software (Invitrogen Inc., CA, USA).
Matched trace files and the corresponding mate pairs were manually assembled using computer software (SeqMan, Lasergene 6.0, Madison, WI).
Each component of ABAleon was manually assembled using COOT (Emsley et al., 2010).
A metabolic reconstruction of human sIECs was manually assembled using the literature.
The delineated pathways were then combined into plausible signalling cascades and initial sub-models were manually assembled using the PathVisio software.
For single section movies, time-lapse imaging datasets were manually assembled using ZEN software, and then the brightness and contrast of movies were adjusted using Fiji.
Similar(54)
The sequences were edited manually and assembled using the Mega 3.1 sequence editor [51].
Resulting sequences were edited manually and assembled using Sequencher software, version 4.0.5 (GeneCodes).
All chromatograms were manually corrected and assembled using the software Sequencher.
Sequences were manually checked and assembled using BioEdit v. 7.0.5.3 [ 40] and then annotated with ARTEMIS v. 9 [ 41].
Sequence traces were imported into Geneious Version 5.6.5 and manually trimmed and assembled using appropriate 'Williams 82' genome sequences as references (ftp://ftp.jgi-psf.org/pub/compgen/phytozome/v9.0/Gmax/).
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