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To our knowledge this is first systematic characterization of the human kinome across major human tissue and cancer types at the transcriptional level, together with functional associations with other transcribed genes.
To identify novel splice variants, we performed RT-PCR using an mRNA pool representing major human tissue types and tumors.
To identify novel splice variants from cell surface receptor genes, we performed RT-PCR using a complex mRNA pool representing major human tissue types and tumors.
mRNAs that represent major human tissue types from healthy or diseased tissues and from cell lines were purchased from Clontech (Mountain View, CA, USA) and from Strategene (La Jolla, CA, USA), and were pooled.
To better characterize the esMCs and esMDMs, we studied the global expression of mRNA transcripts in these two cell types and compared the expression pattern relative to that of primary adult bMCs and several major human tissue samples including spleen, thyroid, brain, breast, heart, kidney, liver, pancreas, prostate, skeletal muscle, and testis.
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Here, we have investigated the genes and proteins with elevated expression in cardiac and skeletal muscle in relation to all other major human tissues and organs using a global transcriptomics analysis complemented with antibody-based profiling to localize the corresponding proteins on a single cell level.
To determine the tissue-specificity of human miRNAs, Hsu et al. detected the expression levels of 224 miRNAs in 18 major human normal tissues by using a real-time PCR-based method [ 39].
The final group of presentations concerned practical experiences of major specimen banking and human tissue monitoring programs in the United States and Europe.
The major function of the enzyme human tissue transglutaminase (TG2) is the crosslinking of proteins via a transamidation between the γ-carboxamide of a glutamine and the ε-amino group of a lysine.
The 18 methylation samples were clustered into 3 major groups, each representing a human tissue.
This motivated us to critically compare and to consolidate four major microarray data sets of human tissue expression due to their outstanding importance for functional genomics and large-scale systems biology.
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large human tissue
severe human tissue
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major human development
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major human influenza
major human problem
major lymphoid tissue
major local tissue
major human impact
major metabolic tissue
major human migration
major connective tissue
major erythropoietic tissue
major peripheral tissue
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