Sentence examples for maintained in physiological from inspiring English sources

The brain was sliced (300 µm) in the coronal plane using a vibratome (Integraslice, Campden Instruments, UK) and maintained in physiological saline at 4°C.

The brain was sliced (300 µm) in the coronal plane using a vibratome (Integraslice, Campden Instruments, Loughborough, UK) and maintained in physiological saline at 4°C.

Another signalling candidate for promoting an increased growth rate was β-catenin, as immunofluorescence microscopy experiments revealed nuclear β-catenin localisation both in NHU cells maintained in low calcium (Figure 3B) and in NHU-ECmut cells maintained in physiological calcium (Figure 3C).

In comparison to the control cells, the growth rate of NHU-ECmut was elevated in low calcium conditions and NHU-ECmut cells maintained in physiological calcium showed virtually identical growth rates to NHU-Con cells cultured in low calcium (Figure 4B); this was in agreement with the results from [3H]-thymidine incorporation assays (Figure 4A).

Cells in G2/M cells were of relatively low frequency, lowest in tissues maintained in physiological buffer, increased in media, and significantly increased after U46619 stimulation (Fig.  4).

To investigate if aberrant hemichannels lead to the EKV mutant-induced cell death phenotype, transfected cells were maintained in physiological or high calcium and cell death was quantified by FACS analysis of PI uptake (dead cells are permeable to PI).

Freshly isolated retinas could be maintained in a physiological environment that can minimize the differences from in situ metabolic status of living retinas.

Chondrocytes are maintained in their physiological microenvironment, the de-differentiation phenomenon is prevented and the inter-individual variables are reduced [ 48].

Vital signs including blood pressure, heart rate, blood oxygenation, body temperature, and CO2 in expired air were monitored with a Surgivet Instrument (Waukesha, WI) and maintained in normal physiological limits.

Thus, proper balance of intracellular NF- κB should be maintained in the physiological conditions, while intricate interactivity between NF- κB and other signaling pathways needs to be extensively investigated.

We address this knowledge gap by evaluating skeletal muscle function in nNOS knockout (KN1) mice using an in situ approach, in which the muscle is maintained in its normal physiological environment.