Small airway epithelial cells (SAECs; Lonza Inc., Walkersville, MD) are human primary pulmonary epithelial cells isolated from distal airspaces; they were maintained in saline-adenine-glucose-mannitol medium supplemented according to the supplier's instructions.
The brain was sliced (300 µm) in the coronal plane using a vibratome (Integraslice, Campden Instruments, Loughborough, UK) and maintained in physiological saline at 4°C.
The brain was sliced (300 µm) in the coronal plane using a vibratome (Integraslice, Campden Instruments, UK) and maintained in physiological saline at 4°C.
Parasites were maintained in normal saline during the experimental period.
For collection of round worms intestines of freshly slaughtered fowls were dissected and were maintained in normal saline solution.
Both oligos were purchased from Gene Tools LLC (Philomath, OR, USA) and maintained in sterile saline according to the manufacturer's instructions.
At various times after injection, mice were killed, brains were surgically removed, and brain tissues were dissected and maintained in cold saline containing protease inhibitors until processing.
Cells were maintained in a saline solution through a laminar-flow perfusion and the chosen field was stimulated after 10 s of baseline acquisition.
The worms were collected after dissection of intestines and maintained in normal saline solution, having an average size of roundworms and tapeworms as 5 7 cm and 6 7 cm, respectively.
Live cells were adherent to collagen-coated coverslips were maintained in imaging saline (125 m M NaCl, 5 m M KCl, 1 m M Na3PO4, 1 m M MgSO4, 20 m M HEPES, 1 m M CaCl2, 5.5 m M Glucose 20 m M) for the duration of experimentation.
