HEK293 cells were maintained in DMEM (Biological Industries, Beit Haemek, Israel) containing 10% FBS (Biological Industries, Beit Haemek Israel) and 1% Pen/strep (100U/ml) (Biological Industries, Beit Haemek, Israel).
Each callus line was maintained in three biological replicates.
Mice were maintained in the Biological Resource unit at the University of Dundee in compliance with UK Home Office Animals Scientificc Procedures) Act 1996 guidelines.
HL-60 and Rin5f were maintained in RPMI (Biological Industries, Beit Haemek Israel) containing 10% FBS and 1% Pen/strep (100U/ml) at 370C in a 5% CO2 humidified atmosphere.
The cultures were usually maintained in QBSF®60 (Quality Biological, Inc., Gaithersburg, MD) containing10% FCS, 100 U/ml penicillin, and 100 mg/ml streptomycin with indicated cytokines.
Rings were embedded in collagen gel and maintained in Bio MpM medium (Biological Industries, Israel).
Human MCF-7 breast adenocarcinoma cells and 501mel human metastatic melanoma cells were maintained in RPMI 1640 medium (Highveld Biological, Johannesburg, South Africa) supplemented with 10% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin.
Ac-AChBP was overexpressed in High Five insect cells maintained in SIM-HF medium (Sino Biological Inc., China) using the Bac-to-Bac baculovirus expression system (Invitrogen, Thermo Fisher Scientific, USA).
Animals were maintained in standard condition at the Biological services Unit, Hammersmith Hospital, London, UK.
