By contrast, ER α expression is stable and can be maintained in cultures of purified malignant epithelial cells.
These selective advantages when coupled to its mode of synthesis and partitioning, insure that EBV is maintained in infected normal and malignant proliferating B-cells.
Since MLH1 was downregulated early and maintained in a low expression level in the NNK induced malignant transformation process, a possible involvement of epigenetic regulation was considered.
Highly malignant mouse +SA mammary epithelial cells were maintained in culture on serum-free defined media containing 10 ng/ml EGF as a mitogen.
The malignant tongue-derived squamous cell carcinoma keratinocytes, SCC25 [12] were maintained in DMEM (Cambrex) supplemented with 10% FCS.
In Taiwan, patients who receive HSCT or have malignant diseases are classified in the 'Catastrophic Illness' group and maintained in a separate NHRI database (Catastrophic Illness Registry).
Non-malignant (spontaneously immortalized HaCaT keratinocytes) and malignant (SCL-1 cells, derived from a cutaneous squamous carcinoma) human keratinocytes were maintained in RPMI 1640 medium (PAA Laboratories) supplemented with 10% fetal calf serum (Biochrom AG) and 1% L-glutamine, as reported previously.
Malignant cancer cells usually have similar properties as embryonic cells characterized with elevated stemness markers and maintained in a dedifferentiated status (Reya et al., 2001).
U87MG malignant glioma cells (ATCC HTB-14) and MDAH 2774 ovarian adenocarcinoma cells (ATCC CRL-10303) were maintained in RPMI-1640 medium supplemented with 10% heat inactivated FBS.
MicroRNAs are dysregulated in most forms of cancer and have a role in maintaining the malignant phenotype via alterations in processes such as cellular adhesion, angiogenesis, cell cycle control and apoptosis (Lu et al, 2005; Dalmay and Edwards, 2006).
