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Exact(6)
Briefly, all cell lines were cultured on Matrigel (BD Biosciences) coated plates and maintained in mouse embryonic fibroblast conditioned medium (MEF-CM) supplemented with 8 ng/ml of human recombinant basic fibroblast growth factor (bFGF, Invitrogen) [41].
Cells were maintained in mouse flank xenografts and processed using a papain dissociation kit (Worthington, Lakewood NJ, USA).
We showed that during 4 weeks of automated culture, the expression of SSEA-1 and SSEA-3 was maintained in mouse iPS cell cultures.
Mice were maintained in mouse metabolic cages (Hatteras Instruments, Cary, NC) during the study under controlled temperature and light conditions (12-h light and dark cycles).
Interestingly, while levels of H3K9me3 are maintained in mouse embryonic fibroblasts deleted for HELLS, dramatic changes in H3K4me and acetylation levels at the pericentromeric heterochromatin have been reported [ 62, 63].
This scenario is supported by the finding that 1st phase insulin secretion evoked by an increase in glucose from 3 to 22 mM is maintained in mouse islets loaded with the fast Ca2+-buffer BAPTA to an estimated intracellular concentration of 1 mM [64].
Similar(54)
Importantly, these data show that varying degrees of increased locomotor activity (in other words, "exercise") can be elicited and maintained in mice by manipulating the schedule of reinforcement.
The non-culturable IOE was maintained in mice.
This H37Rv strain has been maintained in mice to prevent loss of virulence and is not subjected to repeated in vitro cultivation.
EAU was diminished significantly by day 21 P.I. in mice immunized with 25 or 50 mg IRBP peptide but was maintained in mice receiving 100 µg IRBP peptide (Fig. 2).
After a third passage, using mouse brains with "C-BSE like" features, the biochemical and histopathological similarities with classical BSE were confirmed, whereas the characteristics of H-BSE were maintained in mice inoculated with a brain homogenate containing H-type PrPres. H-type PrPres
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