Sentence examples for maintain protein solubility from inspiring English sources

The buffers used for integral membrane proteins contain detergents, which are required to maintain protein solubility.

The arginine was necessary to maintain protein solubility at high concentrations.

At low concentrations, TCH6 was the most active protein tested, but higher levels of the protein were toxic to cells, presumably because of the concentration of NaCl required to maintain protein solubility during preparation of the enzyme.

These additives aid in the separation of the protein from the lipid content, maintain protein solubility, and assist in protein unfolding to maximize the availability of protease cleavage sites.

Fractions containing U2A′ were concentrated using a Vivaspin concentrator with a molecular mass cutoff of 10 kDa and buffer-exchanged into 100 mM arginine, 50 mM KCl, 10 mM cacodylate (pH 7), and 5 mM DTT; arginine was necessary to maintain protein solubility at high concentrations.

For this reason, we were anxious to find conditions that would allow long-term storage of conjugated proteins without loss of either transcriptional or cleavage activities, and since T7 RNAP precipitates if salt concentrations are less than ~0.1 M, we also sought buffer conditions for conjugation which would maintain protein solubility and activity.

However, the resultant chimeric protein aggregated, suggesting that unlike HKI and HKII, the N-terminal region of HKIII may play a role in tertiary structure formation and in maintaining protein solubility.

No reliable fluorescence measurement was obtained for ArntbHLH binding the E-box in Buffer A. We suspect protein misfolding, and possible formation of soluble aggregates, lead to nonfunctional protein, and hence, our use of significant amounts of denaturant that maintains protein solubility, yet decreases the physiological relevance of these experimental conditions.

Amphipols were originally developed to maintain membrane protein solubility after treatment with a nondenaturing detergent.

Despite their key roles in maintaining cellular protein solubility under and following stress conditions, the determinants of sHSP structure and function remain ill defined.

Subfunctions in different subunits (catalysis, regulation, and protein solubility) co-evolved in heterotetramers to maintain physiologically functional enzymes.