Sentence examples for maintain protein activity from inspiring English sources

AqpZ-incorporated polymer vesicles were prepared from disulfide-functionalized poly 2-methyloxazoline -block-poly(dimethylsiloxane)-block-poly 2-methyloxazoline -block-poly-functionalized poly 2-methyloxazoline -block-polycopoly 2-methyloxazoline -block-polyn to be able to maintain protein activity after the incorporation as foundimethylsiloxane -block-poly 2-methyloxazoline dimethylsiloxane -block-poly 2-methyloxazoline dimethylsiloxane -block-poly 2-methyloxazoline dimethylsiloxane -block-poly 2-methyloxazoline

Although we did not test the activity of all the protein polymer constructs, our data supports an emerging wave of examples showing the ability to maintain protein activity in protein polymer bioconjugates prepared using site-specifically incorporated ncAAs.

Model fluorescent proteins were expressed using nonsense codon replacement to incorporate azide-containing unnatural amino acids in a site-specific manner toward maintaining protein activity: here, cyan fluorescent protein (AzCFP), mCherry fluorescent protein (AzmCh), and mCh decorated with a thrombin cut-site.

This provides a gentle immobilisation approach that usually maintains protein activity.

Indeed, PTEN alleles that maintain protein phosphatase activity were able to dephosphorylate this peptide in vitro.

For this reason, we were anxious to find conditions that would allow long-term storage of conjugated proteins without loss of either transcriptional or cleavage activities, and since T7 RNAP precipitates if salt concentrations are less than ~0.1 M, we also sought buffer conditions for conjugation which would maintain protein solubility and activity.

Hence, severe mis-sorting and strong secretion of vacuolar hydrolases might alternatively explain the failure of ESCRT mutants to recycle amino acids, maintain protein synthesis and boost the catabolic activity of vacuoles.

Slp1 has three N-glycosylation sites and that simultaneous N-glycosylation of each site by the α-1,3-mannosyltransferase Alg3 was found to be required to maintain protein stability and the chitin-binding activity of Slp1, which are essential for its effector function (Chen et al. 2014).

Our results show that Runx-dependent activation of SpPKC1 is essential for maintaining protein kinase C activity at levels conducive to cell survival during embryogenesis.

One limitation of this technique, however, is maintaining protein stability and activity during the fabrication process.

For the first time we have demonstrated that using high vacuum electrospray deposition it is possible to deposit large protein molecules on polymeric surfaces whilst maintaining the protein activity.