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The differences in lignin removal and phenolic compound production from native P. trichocarpa x P. deltoides and CEL suggested that lignin-carbohydrate interactions increased lignin extraction and the extractability of syringyl groups relative to guaiacyl groups.

A modified lignin extraction method was employed to extract lignin from different biomass samples for further Py-GC-MS.

To this end, design and development of an eco-efficient lignin extraction method is crucial.

It was also demonstrated lignin extraction from lignocellulosic using recycled IL gave sufficient performance.

The steam-assisted lime treatment resulted in 69.67 (±1.26) % of the lignin extraction the MW-assisted lime treatment resulted in 68.27 (±1.19) % of the lignin extraction from the corresponding autohydrolysed sweet sorghum bagasse samples.

Under optimum conditions, the SAHT resulted in 1.46 (±0.04) % of lignin extraction into the hydrolysate, whereas under optimum conditions for MAHT, the lignin extraction was 3.80 (±0.17) % from SSB.

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Lignin extractions were also performed following Technical Association of Pulp and Paper Industry (2013) standards, by adding 15 mL of 72%% sulfuric acid solution to 1 g of extractive-free wood in a beaker placed in an 18 20 °C water bath for 2 h.

It is possible that during this lignin-extraction process, minor artificial structural modifications would be introduced into the lignin polymeric framework.

Lignin was extracted from dewaxed Acacia wood by alkali extraction method as described previously by Aadil et al. (2014).

The methodology has been applied to biorefining technologies for lignin and hemicelluloses extraction and valorisation, both technologies being coupled with gasification of wood residue.

Moreover, recent research on lignocellulosic feedstock pretreatment with NADES reagents showed high specificity towards lignin solubilisation and extraction of high purity lignin from agricultural residue such as rice straw (Kumar et al. 2016).

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