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Moreover, lignin fractionation allows to obtain lignin fractions with different chemical characteristics and polymerisation capability.
The maximum hemicellulose fractionation yield was 73% in SE, while the maximum lignin fractionation yield was 90% in ethanol extraction.
Furthermore, the higher solubility of lignin fragments in ethanol-based liquors helped to increase the lignin fractionation yield and reduce lignin condensation [ 28].
The corresponding ANOVA value in Table 6 indicated that the order effects of factors on the lignin fractionation yield were temperature (x2) > time (x3) > ethanol concentration (x1).
The optimal conditions of ethanol extraction were 160°C for 2 hours with 60% ethanol (v/v), and the corresponding lignin fractionation yield was 90%.
During the ethanol extraction process, 0.1 to 0.5% (w/w) HCl or NaOH was added in order to improve the lignin fractionation efficiency from SEWS.
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In addition, Kraft dissolved lignin (KDL) was fractionated according to two different protocols (solvent extraction and acidic fractionation) in order to identify possible lignin fractions with noticeable polymerisation ability.
Furthermore, organosolv fractionation prior to alcoholysis allows for the separation and valorisation of the lignin fraction.
Pulp can be used for ethanol production while separated xylan and lignin fractions can be used as a feedstock for value-added products which suggests the applicability of clean fractionation technology in a biorefinery concept.
The most important chemical modifications taken place in the lignin during the fractionation were identified: β-O-4′ cleavage and hydrolysis of LC-bond structures.
These results showed that the anti-solvent was important for biomass fractionation (lignin separation from biomass).
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