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Fruits harvested at the color breaker stage were used for the inoculation experiment.
The inoculation experiment was repeated twice at the second trial (April 2009).
Thereby, we tested two MCCP variants with different initial mineralization activities at the beginning of the soil inoculation experiment.
This field inoculation experiment was conducted at Fedis and Babile sites where the native rhizobia of peanut were 2.6 × 102 and 3.3 × 103 rhizobia of peanut g−1 soil, respectively as estimated by the MPN method.
To validate the reliability of DGE sequencing, the expression of 12 randomly-selected DEGs were revealed by quantitative real time PCR (qRT-PCR) assays in another independent inoculation experiment.
In a previous study, the IR24 NILs (IRBB lines) containing Xa4, xa5, Xa7 and Xa21 genes and their combinations conferred different degrees of resistance to K1, K2, K3 and K3a races in a field inoculation experiment in Korea (Jeung et al.2006; Kim et al.2009; Suh et al.2009a).2009a
Similar(34)
Plants were either cultivated in half MS medium without sucrose and inoculated with bacteria (inoculation experiments) or vacuum-infiltrated with a bacterial solution in 10 mM MgCl2 (infiltration experiments).
At the end of the inoculation experiments, 14C-mass balances were calculated.
All inoculation experiments used an overnight accumulation culture grown to stationary phase in advance.
Three treatments per labeling experiment were established, with each treatment prepared as described for our inoculation experiments.
Plants were cultivated in a greenhouse at 25°C from November to April, and inoculation experiments were conducted in March.
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