For each infection experiment, total RNA samples were obtained from infected and mock-infected cells at 8 hours post-infection and checked for quantity and quality (Nanodrop, Agilent).
An alternative explanation might be that the moi of 3 used to initiate this single-step infection experiment was not sufficient to infect all cells simultaneously.
The Infection experiment was repeated once with group C (infected) and group D (control).
In the infection experiment, three host groups within both selection lines were infected with one of the tree bacterial strains.
The same infection experiment carried out with full-genome replicon cells produced no infectivity in the supernatant (data not shown).
For each infection experiment, a minimum of two rats per inoculum dose were infected, so that each dose was represented in each experiment.
Only results from one infection experiment (34 CFU) was shown.
This infection experiment was carried out in triplicate.
The infection experiment was synchronised for 30 min at 4°C.
Male Mongolian gerbils, two months of age, were used in the leptospira infection experiment.
Colonies used in the infection experiment study were collected from the middle keys, oceanside site.
