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Second, in the postimplantation AND injection experiment, 1 × 10 cellslls were injected in the middle of both ears in ICR mice.
(Note that the elba2 and elba3 dsRNA injections shown here were part of the same injection experiment and have the same buffer injected control).
When injections were performed using as carrier the DNA recovered from the main fraction of the density gradient (GC level around 37.9%), the expression pattern of the injected construct was identical to that observed in a typical injection experiment (and to that of the endogenous spdri gene).
For the dual injection experiment, EdU and BrdU (300 μl, 10 mM each) were sequentially injected at a 4-hr interval.
During mercury injection experiment, due to extremely high pressure the mercury penetrates into very small pores.
A shallow CO2 injection experiment was conducted in an unconfined, unconsolidated siliclastic aquifer in western Denmark.
Okolo et al. (2015) has studied effects of coal compressibility on the pore structure distribution from mercury injection experiment.
The results of one zone compared with the pore size distribution which was derived from mercury injection experiment.
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For co-injection experiment, we injected each embryo twice, first with the MO and then with the mRNA or another MO.
Similar to the data in co-injection experiment described above, the U87-EGFRvIII flank tumors injected with hMSC-scFvEGFRvIII were 1.7 times smaller than tumors in the control (PBS) group (p = 0.03, n = 6) or tumors injected with control hMSCs (p = 0.05, n = 6) (326±79 vs 542±220 vs 555±267 mm3 respectively) at the end of experiment (Figure 3C).
Generally, a small amount of mercury is adsorbed on the surface of rock because of rough rock surface before pressurizing in mercury-injection experiment.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com