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We also examined small RNA fungal profiles in planta at 72 and 96 hours post inoculation (hpi), as well as mock inoculated plants.
Horizontal cross sections (50 μm thickness) of roots (inoculated and non-inoculated) were dissected 24, 48, 72 and 96 h post inoculation (hpi) using a sectioning system (Vibratome 1000 plus) and placed between glass slides in SDW.
The inoculated plants were scored 48 and 72 h post inoculation (hpi) for resistant and susceptible host responses.
Leaf tissues (first-fourth leaves) of ZC108 and LJ43 were sampled at 0 h (before inoculation), 24 and 72 h post inoculation (hpi).
The SA signaling was activated within 12 h post inoculation (hpi) followed by the JA signaling which was activated around 24 hpi.
To define the expression pattern of PsAvr3c during P. sojae infection, we performed quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) at 0.5,1,2,3,6,24 and 36 hours post inoculation (hpi) on susceptible soybean.
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Quantitative RT-PCR (qRT-PCR) showed increased TV RNA replication in MAL-treated compared with untreated cells at 4 hours post-inoculation (HPI) and the increase continued steadily for at least 28 HPI (Fig. 5A).
(D) Conidia-covered Arabidopsis root 6 h post-inoculation (hpi).
The transcript level of OsHsp18.0-CI increased quickly after 6 h post-inoculation (hpi) with RS105 (Fig. 1) and reached maximum level at 24 hpi, with an increase of 16-fold compared with the control.
Transcripts of wheat and barley GER3 and GER4 genes accumulated predominantly in epidermal cells compared to mesophyll cells as early as 6 h post-inoculation (hpi; Wei et al. 1998; Schweizer et al. 1999; Zimmermann et al. 2006).
Appressorium formation rate was counted at 24 hours post-inoculation (hpi) under the microscope, more than 200 appressoria were counted for each strain.
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