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The volume of the injector loop was 20 μl.
The filling of the injector loop with pneumatic syringe decreases losses of the injection solutions compared to the inbuilt fluid detector.
For the RP nano-LC separation, a Tempo LC MALDI Spotting system (Applied Biosystems/MDS SCIEX, Foster City, CA, USA) was used with a 2-μL injector loop and a Chromolith CapRod column (150 × 0.1 mm; Merck, Darmstadt, Germany).
For reverse phase nanoflow-LC, each SCX fraction was autoinjected onto a Chromolith CapRod column (150 × 0.1 mm; EMD Chemicals Inc., Gibbstown, NJ) using a 5 μl injector loop on a Tempo LC MALDI Spotting system (ABI-MDS/Sciex).
The analysis was performed on an Agilent 1100 series HPLC system (Agilent Technologies, USA) equipped with a quaternary pump, a vacuum degasser, a column thermostat, a 20 μL injector loop, a UV detector and HP ChemStation software.
Similar(54)
The HPLC operations were modified by installing pneumatic column selector and a syringe operated loop injector.
The HPLC system consisted of a Waters 600E Multisolvent Delivery system, Waters In-Line degasser AF, a Rheodyne injector with sample loop of 20 μl (180 μg of the extract), and a Waters 2669 photodiode array (PDA) detector.
Measurements were made with a Jasco (Mary's Court, Easton, MD, USA) liquid chromatograph equipped with UV and diode array (DAD) detector, an injector with a loop of 50 μL, a quaternary pump, a vacuum degasser an a thermostated column compartment.
For the high pressure injector, a closed-loop control of the needle opening duration enables the injection of extremely small quantities.
The analytical system used consisted of a 1525 μ binary HPLC pump, 2996 PDA detector, 1500 series column heater, and in-line degasser and a Rheodyne 7725i manual injector with 20-μl loop (Waters Corporation, Milford MA).
The analytical system used consisted of a 1525 μ binary HPLC pump, 2996 PDA detector, 1500 series column heater, in-line degasser and a Rheodyne 7725i manual injector with 20 μL loop (Waters Corporation, Milford MA).
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