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A transwell filter assay was employed to further analyze tumor cell migration [32].
Moreover, although straightforward to perform with the filter assay, reliable controls are often not done.
We have developed an easy-to-use two dot visual filter assay for translation into current water testing in public health laboratories to detect E. coli O157 H7.
Results show, for example, that in the filter assay, 2 4 times as many neutrophils pass through the filter when exposed to a gradient as when the gradient is absent.
Amyloid burden was estimated biochemically by filter assay as previously described [32].
The filter assay for the detection of the aggregates was performed as described previously [8].
Similar(13)
SD/-Trp-His-Ade screening combined with β-galactosidase filter assays using X-gal was performed to test autonomous activation.
For selection purposes, β-Galactosidase (β-Gal) filter assays were performed using X-Gal as substrate according to the manufacturer's instructions (Clontech).
Out of 123 initial prey clones obtained, 39 clones remained positive following pre-selection on selective growth media and β-Gal filter assays.
Colonies were additionally filter assayed for β-galactosidase activity.
The individual DNA samples used in the filter assays were also used in these assays.
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