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Filter sterilize.
Filter sterilize the stock solution.
Dissolve each component completely in order listed and then filter sterilize.
3. Quickly weigh streptozotocin, dissolve in cold citrate buffer, and filter sterilize through a 22-μm pore filter.
Briefly, adenoviral lysates from twenty-four 150 mm plates were banded twice on CsCl gradients and desalted twice with a PD-10 size exclusion column (Amersham Scientific, Piscataway, NJ) into HEPES buffered saline (HBS; 21 mM HEPES, 140 mM NaCl, 5 mM KCl, 0.75 mM Na2HPO4·2H2O, and 0.1% (w/v) dextrose; adjust pH with NaOH to 7.5; and filter sterilize) containing 5% glycerol, and stored at -70°C.
Keep your scent sprays and wafers inside the bag (I boil down a gallon of leaves/needles/detritus from within 1000 yards of the space I'll be hunting, strain with a coffee filter, sterilize with rubbing alcohol and use it for a scent spray).
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Filter sterilized vitamins were added after heat sterilization of this medium.
The final material was filter sterilized, aseptically vialed, and stored at −80 °C.
Juice was filter sterilized with a 0.2 μm membrane filter (Sartorius Australia, Oakleigh, Victoria, Australia).
Hydrolysates were always filter sterilized (0.2 μm, Millipore, USA) prior to inoculation.
Prior to the mutagenicity assay, test samples were filter sterilized by passing through 0.22 µm filters to remove turbidity.
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