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A sensitive screening assay for deficient fibrinolytic activity in vivo.
The remainder of the confirmatory assay procedure was the same as for the screening assay.
Open image in new window Fig. 2 Scheme of the high-throughput screening assay.
The remainder of the assay procedure was the same as the screening assay.
Our screening assay did not find a preferable amino acid exchange at this position.
Such a screening assay has not yet been described for AEHs.
A newly developed ELVIRA HSV screening assay was used that allowed a high throughput screening.
Such a screening assay has not yet been described for GPs.
Control experiment using a commercial inhibitor of acetylcholinesterase, neostigmine methyl sulfate (IC50 ≈ 40 nM), was included in each screening assay.
In our anti-EV71 drug screening assay, RD cells were seeded at 5 × 105 cells/well in 6-well plates.
Open image in new window Fig. 3 The results of the high-throughput screening assay represented as a heatmap.
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