[3H]-triptolide was prepared and a filtration assay designed to measure binding to cells and cellular extracts.
Five diagnostic procedures were used: direct smear examination, the Knott's modified test, filtration assay, and two heartworm antigen detection kits.
In this regard, the combination of filtration assay with QDs-labeled probe can offer great advantages over enzyme-based and other kinds of fluorescence-based analytical formats.
In this novel screen, we have coupled gene knockdown using morpholinos with a physiological glomerular dye filtration assay to test for selective glomerular permeability in living zebrafish larvae.
Recently, our gel filtration assay has revealed that OsRac1 forms a large protein complex containing those OsRac1 interactors (Chen et al. 2010a) (S. Hamada, and K. Shimamoto, unpublished data).
By using this method, we found that CLE42 was co-purified with the PXL2LRR protein in the gel filtration assay, suggesting that CLE42 may act as a ligand of PXL2 (Fig. 1B).
Using the gel filtration assay, we calculated the molecular weights of His-tagged recombinant CYN proteins.
In initial efforts to measure levels of circulating FRα by 3H-FA binding studies, preliminary studies were done to determine the attributes of the microcentrifuge filtration assay.
