Sentence examples for downstream sequencing from inspiring English sources

To surmount this problem in a cost-effective manner, we linearly amplified all mRNA species by adapting template switching PCR, allowing us to extract 5 to 10 μg of amplified polyA-tailed mRNA from 1 ml of blood, sufficient for downstream sequencing or microarray analysis.

This cloning system allows induction of the fosmid copy number to ∼50 copies/cell, which was critical to simplification of library screening and downstream sequencing.

These represent a library that is suitable for downstream sequencing reactions [ 8].

Liquid cultures were purified for downstream sequencing using a QIAprep Spin Miniprep kit (Qiagen).

Throughout library preparation, cDNA was QC validated for downstream sequencing with the Agilent Bioanalyzer.

Downstream sequencing is minimised as dMS-HRM acts as a screen to select only methylated clones for further analysis.

The processing and transport of essential elements follow a downstream sequence.

P dnaK prolonged to the downstream sequence of initiation coden and carried additional optimal RBS and AS of R4a.

The upstream and downstream sequences of the nprE gene were inserted into plasmid pUK, yielding nprE knockout vector pUNprEK.

To further characterise the genetic locus of this major virulence factor, we analysed the ptx/ptl upstream and downstream sequences.

After Illumina sequencing, downstream sequence analysis was performed using Mothur software (http://www.mothur.org) based on standard procedure (Schloss et al. 2009).