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Other technologies are in development that could sharply lower sequencing costs.
"We believe Incyte has substantially lower sequencing capacity than Celera," says Gibbons.
A new nucleic acid sequencing platform, the Sequel System, provides higher throughput, more scalability, a reduced footprint, and lower sequencing project costs compared to the PacBio RS II System, while maintaining the existing benefits of single molecule, real-time (SMRT) technology.
Even at lower sequencing depths, sequence-derived allele frequencies were highly predictive of the actual frequencies, as estimated from genotyping the individuals in the pools (Figure 5).
This discrepancy might be explained by a lower sequencing effort done here but also by the selective nature of anoxic waters that might impose stronger constraints on the communities compared with open ocean conditions, implying a lower diversity and therefore a higher overlap between rDNA and rRNA libraries.
It may attribute to the lower sequencing depth.
Thus, a relatively lower sequencing throughput is required, compared with bisulfite-sequencing.
We next asked if f could be accurately estimated using a lower sequencing depth.
However, our targeted approach efficiently enriched for genes that were targeted, at considerably lower sequencing depths.
This technology created a balance between higher genotyping accuracy and relatively lower sequencing cost.
Sites with mismatched genotypes have on average lower sequencing coverage than sites with matched genotypes.
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