Downstream promoter element.
As expected, gp2 greatly decreased RNAP affinity to downstream promoter duplex.
However, gp2 also inhibited RNAP binding to promoter fragments that lacked downstream promoter DNA that interacts with the β′ jaw.
The OfC1Inh had a TATA-deficient promoter with a putative initiator element, and two tandemly arranged downstream promoter elements.
gp2 inhibits formation of open promoter complex by binding to the β′ jaw, an RNAP domain that interacts with downstream promoter DNA.
We set out to determine the factors necessary for downstream promoter element (DPE -dependent transcription anDPE -dependentgainstranscriptions, TFIID and the GTfindre nothatfficient.
Of the two SNPs in the downstream promoter region (S886 and S1267), one was in the consensus GT-1 binding site and the other one in the ACGT sequence.
About 30-nt downstream of Inr is the Downstream Promoter Element (DPE), which is recognized by the TBP-associated factor (TAF) to enhance TBP binding to the core promoter (Burke and Kadonaga, 1997).
In addition to the TATA-box, the composite prototype model of metazoan core promoter shows all previously identified cis-elements (BRE= TFIIB recognition element; DPE= Downstream promoter element; Inr= Initiator sequence) occurring in various combinations with the TATA-box.
All SNPs identified in the OsHKT1 4 gene were associated with SPAD chlorophyll content, among them, two were in the downstream promoter region while eight were in the intronic region.
Both upstream and downstream promoter regions appeared to have an additive effect on mRNA expression.
