A DNA construct encoding this hybrid-receptor was inserted into a baculoviral expression vector and expressed in Sf9-cells.
The aim of this study is to further optimize the DNA construct to achieve preventive and therapeutic efficacies.
Briefly, 2 µg of IL-6-luciferase DNA construct was transfected.
The sequence and orientation of the DNA construct was verified by DNA sequencing.
Four founder transgenic fish carrying the EGFP-zProgerin DNA construct were thus identified.
IRF2 monocistronic RNA was generated from the corresponding DNA construct (Fig. 1A).
The DNA construct of the transgene was confirmed by DNA sequencing (data not shown).
The dicistronic DNA construct has been used as the major assay to test for IRES activity.
Cells were transfected with 2 µg each DNA construct using Lipofectamine2000 (Invitrogen), according to instructions supplied by the manufacturer.
Moreover, a shorter overall DNA construct increases the entropic force exerted by bead wall avoidance, again discouraging looping [71].
The C-terminally tagged rat TRPV1ε DNA construct was prepared in the pMTH plasmid vector as described [5].
