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These are plants with: silenced cinnamyl alcohol dehydrogenase (CAD) gene; overexpression of polygalacturonase (PGI); and expression of three genes construct containing β-ketothiolase (phb A), acetoacetyl-CoA reductase (phb B), and poly-3-hydroxybutyric acid synthase (phb C).
This allows one to delete genes, construct translational fusions, or even construct specific mutant alleles.
No excision products were detected in either strain that contained only one of the two Ping genes (construct c or d).
A public website for the resource (http://flypush.imgen.bcm.tmc.edu/pscreen/rmce ) containing all of the information about the MiMIC lines: insertion sites, associated genes, construct used for tagging, complementation data and images of brain expression patterns.
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To select for the continued presence of the plasmids, carrying the required customized version of the genes constructed, minimal drop out media were used.
Of note, 9 of the other 22 genes constructed in the network were not spotted on the microarray.
The GFP gene construct was designed to express ubiquitously.
Figure 3 Gene construct of Os04g27430 from the Rice Genome Annotation Database (http://rice.plantbiology.msu.edu).msu.edu
Immunohistochemistry using anti-BMP-4 antibody demonstrated the translation of the introduced gene construct.
The gene construct submitted to GenBank was given the accession No: KX022510.
Subcellular localization of Dm-AMP1 and Rs-AFP2 in plants transformed with polyprotein gene construct was analyzed immunohistochemically.
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