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Exact(4)
A pseudomolecule is a sequencing construct that ideally corresponds to the chromosomal DNA molecule.
Primers were designed to amplify the full sequencing construct in a single PCR reaction.
After confirmation by sequencing, construct pET15b- cp25l and the empty control vector pET15b were transformed for expression into chemically competent E. coli BL21 DE3) cells (Invitrogen).
To identify novel miRNAs we developed a pipeline which incorporates a hidden Markov model to extract the actual cDNA from the sequencing construct, non-heuristic mapping of the reads to the genome allowing both sequence variation and mapping to several places in the genome, and a support vector machine to score predicted hairpins.
Similar(55)
The non-normalized frameshift efficiencies for the native sequence construct in all biological replicates (n = 9) was 5.4 ± 0.9%.
Hong builds moments of extraordinary romantic power, culminating in a brilliant sequence, constructed from a single ten-minute shot parsed with brisk and assertive zooms and pans, in which Young-hee reflects on her bitter experiences.
Fig. 5 Scenario A. Stream sequences constructed by ASNSSS.
We tried to revise the original spliced sequences constructed at seven sites drilled during Exp.
After extensive efforts to replace the disturbed, missed, or duplicated intervals found in spliced sediment sequences constructed onboard during IODP Exp.
The stream sequences constructed by the proposed algorithm as described in Section 5 are highlighted in the tree.
Occasionally, complete consensus sequences constructed from 454 contigs were used to substitute missing BAC sequences.
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