Sentence examples for rna construct from inspiring English sources
In order to evaluate the respective roles of ATP and RNA in activation of the RIG-I RNA complex, we measuRIG-I RNAicomplex constant for ATP (Km,ATP) at saturating RNA (500 nM) and the Michaelis constant for RNA (Km,RNA) at saturating ATP (5 mM) for each RNA construct (kcat, Km,ATP and Km,RNA summary in supplementary Table S4 online).
We have engineered a simple catalytic system in which two short RNA molecules can catalyze their own ligation to form a larger RNA construct.
Understanding the requirements that enable RNA construct binding properties, especially at the hairpin loop or internal bulge, would afford potential therapeutic approaches to control the BIV life cycle.
When this, wt chimeric RNA construct was introduced into a mammalian cell line, along with the M1 substrate, encoding DNA, very significant (67%) intracellular down regulation in the levels of target RNA was, observed.
The box shows the SHAPE handles in the RNA construct.
All transduced cells were maintained separately according to the short hairpin RNA construct used for transduction.
To test this hypothesis Bim and Puma were knocked down using lentiviral small hairpin RNA constructs inducing RNA interference.
Loehrer and Schaffrath [ 22] isolated P. pachyrhizi haustoria, extracted RNA, constructed a cDNA library, and sequenced the whole haustorial transcriptome, resulting in 111,440 de-novo assembled contigs.
We previously successfully used these small hairpin RNA constructs to generate breast cancer cell clones with effectively silencing of RAD21 gene (Xu et al, 2011b).
The small hairpin RNA constructs against Src (number 1 sense sequence, 5′-GCTCGGCTCATTGAAGACAAT-3′ number 2 sense sequence, 5′-GACAGACCTGTCCTTCAAGAA-3′) used in this study were from the BioMedical Genomics Center at the University of Minnesota.
Recent results indicate that transgenic tomato plants expressing a non-infectious PSTVd hairpin-RNA construct exhibit resistance to PSTVd infection; this resistance is correlated with high accumulation of hairpin-derived siRNAs that appear, therefore, to effectively target the mature viroid RNA [67].
